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Brilliant Green Agar with Sulfadiazine

[所屬分類:培養基配方] [發布時間:2021-11-5] [發布人:網站管理員2] [閱讀次數:] [返回]
Brilliant Green Agar with Sulfadiazine

山東拓普生物工程有限公司(si) 培(pei)養基配(pei)方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
Lactose..............................................................10.0g
Sucrose..............................................................10.0g
Pancreatic digest of casein..................................5.0g
Peptic digest of animal tissue .............................5.0g
NaCl....................................................................5.0g
Yeast extract........................................................3.0g
Phenol Red........................................................0.08g
Sulfadiazine ......................................................0.08g
Brilliant Green ..............................................0.0125g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es.
Use: For the selective detection of Salmonella in foods,
especially from egg products. Salmonella other than
Salmonella typhi appear as red/pink colonies surround
ed by a zone of red in the agar indicating nonlactose/su
crose fermentation. Proteus or Pseudomonas species
may appear as small red colonies. Lactose- or sucrose
fermenting bacteria appear as yellow-green colonies
surrounded by a zone of yellow-green in the agar.
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