培養基配方

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Brilliant Green Agar, Modified

[所屬分類:培養基配方] [發布時間:2021-11-5] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工(gong)程有限公(gong)司培養基(ji)配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................12.0g

Lactose..............................................................10.0g

Sucrose..............................................................10.0g

Beef extract.........................................................5.0g

Peptone ...............................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................3.0g

Na2HPO4.............................................................1.0g

NaH2PO4.............................................................0.6g

Phenol Red........................................................0.09g

Brilliant Green .................................................4.7mg

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Do

not autoclave. Cool to 45°–50°C. Addition of 1.0g of

sodium sulfacetamide and 250.0mg of sodium man

delate enhances inhibition of contaminating microor

ganisms. Pour into sterile Petri dishes.

Use: For the selective isolation of Salmonella other

than Salmonella typhi from food and dairy products.

Salmonella other than Salmonella typhi appear as

red/pink/white colonies surrounded by a zone of red

in the agar, indicating nonlactose/sucrose fermenta

tion. Proteus or Pseudomonas species may appear as

small red colonies. Lactose- or sucrose-fermenting

bacteria appear as yellow-green colonies surrounded

by a zone of yellow-green in the agar.