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培養基配方
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Brigg’s Liver Tomato Broth
[所屬分類:培養基配方] [發布時間:2021-11-5] [發布人:網站管理員2] [閱讀次數:] [返回]
Brigg’s Liver Tomato Broth
山東拓(tuo)普生物(wu)工程有(you)限公(gong)司 培養基(ji)配方(fang) //ljxinglong.com
Composition per liter:
Glucose .............................................................20.0g
Neopeptone .......................................................15.0g
Yeast extract........................................................6.0g
NaCl....................................................................5.0g
Tween 80.............................................................1.0g
Soluble starch......................................................0.5g
L-Cysteine·HCl ...................................................0.2g
Tomato juice ............................................... 400.0mL
Liver extract.................................................. 75.0mL
pH 5.0 ± 0.2 at 25°C
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. (Note:
3.0g of proteolysed liver may be used instead of the liv
er extract.) Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Lactobacillus acetotolerans,
a bacterium found in fermented vinegar broth.



