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培養基配方

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Brain Heart Infusion Agar (BHI Agar) (BAM M24 Medium 1)

[所屬分類:培養基配方] [發布時間:2021-11-5] [發布人:網站管理員2] [閱讀次數:] [返回]

Brain Heart Infusion Agar

(BHI Agar)

(BAM M24 Medium 1)

山東拓普生物(wu)工程有(you)限公司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Beef heart infusion..........................................250.0g

Calf brain infusion ..........................................200.0g

Agar ..................................................................13.5g

Proteose peptone...............................................10.0g

NaCl....................................................................5.0g

Na2HPO4·12H2O.................................................2.5g

Glucose ...............................................................2.0g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation of a variety of fastidious and

nonfastidious aerobic and anaerobic microorganisms.