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培養基配方

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Bovine Serum Albumin Tween 80 Broth (BSA Tween 80 Broth)

[所屬分類:培養基配方] [發布時間:2021-11-5] [發布人:網站管理員2] [閱讀次數:] [返回]

Bovine Serum Albumin

Tween 80 Broth

(BSA Tween 80 Broth)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Basal medium ............................................. 900.0mL

Albumin supplement................................... 100.0mL

pH 7.4 ± 0.2 at 25°C

Basal Medium:

Composition per liter:

Na2HPO4.............................................................1.0g

NaCl....................................................................1.0g

KH2PO4...............................................................0.3g

Glycerol (10% solution) ................................. 1.0mL

NH4Cl (25% solution) .................................... 1.0mL

Sodium pyruvate (10% solution).................... 1.0mL

Thiamine (0.5% solution ................................ 1.0mL

Preparation of Basal Medium: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Adjust pH to 7.4. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Albumin Supplement:

Composition per 100.0mL:

Bovine albumin.................................................10.0g

Tween 80 (10% solution) ............................. 12.5mL

FeSO4 (0.5% solution).................................. 10.0mL

MgCl2-CaCl2 solution..................................... 1.0mL

Cyanocobalamin (0.02% solution) ................. 1.0mL

ZnSO4 (0.4% solution) ................................... 1.0mL

Preparation of Albumin Supplement: Add

components to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Adjust pH to

7.4. Filter sterilize.

MgCl2-CaCl2 Solution:

Composition per 100.0mL:

CaCl2·2H2O ........................................................1.5g

MgCl2·6H2O .......................................................1.5g

Preparation of MgCl2-CaCl2 Solution: Add

components to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly.

Preparation of Medium: To 900.0mL of cooled,

sterile basal medium, aseptically add 100.0mL of

sterile albumin supplement. Mix thoroughly. Asepti

cally distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Leptospira

species.

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Bovine Serum Albumin Tween 80 Broth (BSA Tween 80 Broth)