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培養基配方
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Bismuth Sulfite Agar
[所屬分類:培養基配方] [發布時間:2021-11-3] [發布人:網站管理員2] [閱讀次數:] [返回]
Bismuth Sulfite Agar
山東拓普生物(wu)工程有限(xian)公司 培養(yang)基配(pei)方 //ljxinglong.com
Composition per liter:
Agar ..................................................................20.0g
Bi2(SO3)3 ............................................................8.0g
Pancreatic digest of casein..................................5.0g
Peptic digest of animal tissue .............................5.0g
Beef extract.........................................................5.0g
Glucose ...............................................................5.0g
Na2HPO4.............................................................4.0g
FeSO4·7H2O........................................................0.3g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems and Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly and heat with frequent agitation until
boiling. Boil for 1 min. Do not autoclave. Cool to
45°–50°C. Pour into sterile Petri dishes while gently
shaking flask to disperse precipitate. Use plates the
same day as prepared.
Use: For the selective isolation and identification of
Salmonella typhi and other enteric bacilli. Salmonel
la typhi appears as flat, black, “rabbit-eye” colonies
surrounded by a zone of black with a metallic sheen.



