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Bile Esculin Agar

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Bile Esculin Agar

山(shan)東拓普生(sheng)物(wu)工程(cheng)有限公司培養基配方 //ljxinglong.com

Composition per liter:

Oxgall ...............................................................20.0g

Agar ..................................................................15.0g

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

Esculin ................................................................1.0g

Ferric citrate........................................................0.5g

Horse serum.................................................. 50.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid and BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept horse serum, to distilled/deionized water and

bring volume to 950.0L. Mix thoroughly and heat

with frequent agitation until boiling. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 50.0mL of filter sterilized horse se

rum. Distribute into sterile Petri dishes or test tubes.

Cool tubes in a slanted position.

Use: For differentiation between group D strepto

cocci and nongroup D streptococci. To differentiate

members of the Enterobacteriaceae, particularly

Klebsiella, Enterobacter, and Serratia, from other

enteric bacteria. To differentiate Listeria monocyto

genes. Bile tolerance and esculin hydrolysis (seen as

a dark brown to black complex) are presumptive for

enterococci (group D streptococci).

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