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Baird-Parker Agar, Supplemented

[所屬分類:培養基配方] [發布時間:2021-11-3] [發布人:網站管理員2] [閱讀次數:] [返回]
Baird-Parker Agar, Supplemented

山(shan)東(dong)拓(tuo)普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................17.0g
Glycine..............................................................12.0g
Sodium pyruvate...............................................10.0g
Pancreatic digest of casein................................10.0g
Beef extract.........................................................5.0g
LiCl.....................................................................5.0g
Yeast extract........................................................1.0g
RPF supplement.......................................... 100.0mL
pH 7.0 ± 0.2 at 25°C
RPF Supplement:
Composition per 100.0mL:
Bovine fibrinogen .............................................3.75g
Trypsin inhibitor ............................................25.0mg
K2TeO3...........................................................25.0mg
Rabbit plasma ............................................... 25.0mL
Caution: Potassium tellurite is toxic.
Preparation of RPF Supplement: Add compo
nents to distilled/deionized water and bring volume
to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept RPF supplement, to distilled/deionized water
and bring volume to 900.0mL. Mix thoroughly. Gen
tly heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Asepti
cally add 100.0mL of filter-sterilized RPF supple
ment. Mix thoroughly but gently. Pour into sterile
Petri dishes.
Use: For the selective isolation and enumeration of
coagulase-positive staphylococci from foods.
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