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培養基配方
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BAGG Broth (Buffered Azide Glucose Glycerol Broth)
[所屬分類:培養基配方] [發布時間:2021-11-3] [發布人:網站管理員2] [閱讀次數:] [返回]
BAGG Broth
(Buffered Azide Glucose Glycerol Broth)
山東拓普生物工程(cheng)有(you)限公司(si) 培養基配方 //ljxinglong.com
Composition per liter:
Pancreatic digest of casein................................10.0g
Peptic digest of animal tissue ...........................10.0g
Glucose ...............................................................5.0g
NaCl....................................................................5.0g
K2HPO4...............................................................4.0g
KH2PO4...............................................................1.5g
NaN3 ...................................................................0.5g
Bromcresol Purple ..........................................0.015g
Glycerol .......................................................... 5.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Caution: Sodium azide is toxic. Azides also react
with metals and disposal must be highly diluted.
Preparation of Medium: Add 5.0mL of glycerol
to 900.0mL of distilled/deionized water. Add remain
ing components and bring volume to 1.0L. Mix thor
oughly. Gently heat and bring to boiling. Distribute
into tubes in 10.0mL volumes. Autoclave for 15 min
at 10 psi pressure–116°C.
Use: For the cultivation of fecal streptococci. It is
recommended for qualitative presumptive and con
firmatory tests for fecal streptococci.
