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培養基配方

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Azide Blood Agar

[所屬分類:培養基配方] [發布時間:2021-11-2] [發布人:網站管理員2] [閱讀次數:] [返回]

Azide Blood Agar

山東(dong)拓普(pu)生物工程有限(xian)公(gong)司培養基配(pei)方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue..............................5.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

NaN3 ...................................................................0.2g

Sheep blood, defibrinated ............................. 50.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components, ex

cept sheep blood, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45–50°C. Aseptically

add 50.0mL of sterile defibrinated sheep blood. Pour

into sterile Petri dishes or distribute into sterile tubes.

Allow tubes to cool in a slanted position.

Use: For the isolation and differentiation of strepto

cocci and staphylococci from specimens containing

mixed flora.

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