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培養基配方

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APT Broth

[所屬分類:培養基配方] [發布時間:2021-11-2] [發布人:網站管理員2] [閱讀次數:] [返回]

APT Broth

山東拓普生物工程有限(xian)公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Glucose .............................................................10.0g

Yeast extract........................................................7.5g

NaCl....................................................................5.0g

KH2PO4...............................................................5.0g

Sodium citrate.....................................................5.0g

Na2CO3 .............................................................1.25g

MgSO4·7H2O ......................................................0.8g

MnCl2·4H2O .....................................................0.14g

FeSO4·7H2O......................................................0.04g

Polysorbate 80.....................................................0.2g

pH 7.7 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi—118°–121°C.

Use: For the cultivation of lactic acid bacteria. For

the cultivation of heterofermentative lactobacilli

from meat and other foods. The American Public

Health Association recommends adding 100.0μg/L

of thiamine to this medium.