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培養基配方

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Apple Juice Yeast Extract Medium (AJYE Medium)

[所屬分類:培養基配方] [發布時間:2021-11-2] [發布人:網站管理員2] [閱讀次數:] [返回]

Apple Juice Yeast Extract Medium

(AJYE Medium)

山東拓(tuo)普生物(wu)工程有(you)限公(gong)司培(pei)養基配方 //ljxinglong.com

Composition per 1200.0mL:

Agar ..................................................................30.0g

Yeast extract......................................................10.0g

Apple juice......................................................... 1.0L

pH 4.8 ± 0.2 at 25°C

Preparation of Medium: Add yeast extract to

1.0L of apple juice. Mix thoroughly. Adjust pH to

4.8. Autoclave for 10 min at 9 psi pressure–114°C.

Cool to 45°–50°C. In a separate flask, add agar to

200.0mL of distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically combine the

sterile apple juice solution with the sterile agar solu

tion. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the cultivation of Zymomonas species.