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培養基配方

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Aolpha Medium

[所屬分類:培養基配方] [發布時間:2021-11-1] [發布人:網站管理員2] [閱讀次數:] [返回]

Aolpha Medium

山(shan)東拓普生物工程有(you)限公司培養基配方 //ljxinglong.com

Composition per 1041.0mL:

NaCl................................................................100.0g

Agar ..................................................................15.0g

MgSO4·7H2O ......................................................9.5g

MgCl2·6H2O .......................................................5.0g

KCl......................................................................5.0g

Peptone................................................................5.0g

Yeast extract........................................................1.0g

CaCl2·2H2O.........................................................0.2g

(NH4)2SO4...........................................................0.1g

KNO3 ..................................................................0.1g

Metals solution.............................................. 20.0mL

Phosphate solution ........................................ 20.0mL

Vitamin solution.............................................. 1.0mL

pH 7.0 ± 0.2 at 25°C

Metals Solution:

Composition per liter:

MgSO4·7H2O ....................................................29.7g

Nitrilotriacetic acid ...........................................10.0g

CaCl2·2H2O.........................................................3.3g

FeSO4·7H2O...................................................99.0mg

Na2MoO4·2H2O .............................................12.7mg

Metals “44”................................................... 50.0mL

Preparation of Metals Solution: Solubilize ni

trilotriacetic acid with KOH. Dissolve remaining in

gredients. Adjust pH to 7.2 with KOH or H2SO4.

Autoclave for 15 min at 15 psi pressure–121°C. Add

aseptically to sterile basal medium.

Metals “44”:

Composition per 100.0mL:

ZnSO4·7H2O .......................................................1.1g

FeSO4·7H2O........................................................0.5g

EDTA................................................................0.25g

MnSO4·7H2O..................................................0.154g

CuSO4·5H2O.....................................................0.04g

Co(NO3)2·6H2O ..............................................0.025g

Na2B4O7·10H2O..............................................0.018g

Preparation of Metals “44”: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Add aseptically to sterile bas

al medium.

Phosphate Solution:

Composition per liter:

K2HPO4...............................................................2.5g

KH2PO4...............................................................2.5g

Preparation of Phosphate Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Add aseptically to sterile

basal medium.

Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ..............................................10.0mg

Calcium pantothenate ......................................5.0mg

Nicotinamide....................................................5.0mg

Riboflavin ........................................................5.0mg

Thiamine·HCl ..................................................5.0mg

Biotin ...............................................................2.0mg

Folic acid .........................................................2.0mg

Cyanocobalamin ..............................................0.1mg

Preparation of Vitamin Solution: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Filter sterilize and add asep

tically to sterile basal medium.

Preparation of Medium: Add components—ex

cept Metals “44”, phosphate solution, and vitamin

solution—to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Adjust pH of basal medium to 7.0. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°C and aseptically add the Metals “44”, phosphate,

and vitamin solutions.

Use: For the cultivation and maintenance of Ha

lomonas meridiana and other Halomonas species.

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Aolpha Medium