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培養基配方

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Andrade’s Broth

[所屬分類:培養基配方] [發布時間:2021-11-1] [發布人:網站管理員2] [閱讀次數:] [返回]

Andrade’s Broth

山東拓普生物(wu)工(gong)程(cheng)有限公司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of gelatin...............................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Andrade’s indicator....................................... 10.0mL

Carbohydrate solution................................... 50.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems, in tubes contain

ing adonitol, arabinose, cellobiose, glucose, dulcitol,

fructose, galactose, inositol, lactose, maltose, manni

tol, raffinose, rhamnose, salicin, sorbitol, sucrose,

trehalose, or xylose.

Andrade’s Indicator

Composition per 100.0mL:

NaOH (1N solution)...................................... 16.0mL

Acid Fuchsin.......................................................0.1g

Preparation of Andrade’s Indicator: Add

Acid Fuchsin to NaOH solution and bring volume to

100.0mL with distilled/deionized water.

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Adonitol, arabinose, cellobiose,

glucose, dulcitol, fructose, galactose, inositol, lac

tose, maltose, mannitol, raffinose, rhamnose, salicin,

sorbitol, sucrose, trehalose, xylose, or other carbohy

drates may be used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

carbohydrate solution, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat and

bring to boiling. Distribute in 10.0mL volumes into test

tubes containing inverted Durham tubes. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 25°C. Add

0.5mL of sterile carbohydrate solution to each tube.

Caution: Acid Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Use: For the determination of carbohydrate fermenta

tion reactions of microorganisms, particularly mem-

bers of the Enterobacteriaceae. A Durham tube is used

to collect gas produced during the fermentation reac

tion. Acid production is indicated by a pink color.

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Andrade’s Broth