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培養基配方

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Anaerobe Medium (LMG Medium 41)

[所屬分類:培養基配方] [發布時間:2021-11-1] [發布人:網站管理員2] [閱讀次數:] [返回]

Anaerobe Medium

(LMG Medium 41)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar Base.................................................... 800.0mL

Solution A................................................... 100.0mL

Solution B ................................................... 100.0mL

pH 6.9 ± 0.2 at 25°C

Agar Base:

Composition 800.0mL:

Tryptone..............................................................5.0g

Yeast extract........................................................5.0g

(NH4)2SO4 ................................................... 0.5g

MgSO4·7H2O ............................................... 0.1g

Sodium thioglycolate..........................................0.5g

Agar ................................................................... 0.5g

Fe(NH4)2(SO4)2·6H2O ........................... 55.0mg

Na2MoO4·2H2O....................................... 2.4mg

Na2SeO3·5H2O ...................................... 0.23mg

Preparation of Agar Base: Add components to

distilled/deionized water and bring volume to

800.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C.

Solution A:

Composition 100.0mL:

Glucose .............................................................18.0g

K2HPO4 ....................................................... 7.0g

KH2PO4 ....................................................... 5.5g

Preparation of Solution A: Add components to

100.0mL of distilled/deionized water. Mix thoroughly.

Filter sterilize.

Solution B:

Composition 100.0mL:

NaHCO3 .................................................... 10.0g

Preparation of Solution B: Add components to

100.0mL of distilled/deionized water. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Aseptically add solu

tions A and B to the sterila Agar Base. Adjust

pH to 6.9. Mix thoroughly. Distribute into sterile

tubes. Incubate anaerobically under 100% CO2 gas

atmosphere.

Use: For the cultivation of anerobic bacteria.

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Anaerobe Medium (LMG Medium 41)