99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開
個人中心 我的訂單 購物車
編號：
批號：
培養基配方
您現在的位置： 網站首頁 >> 培養基配方

Agar Medium for Differential Enumeration of Lactic Streptococci

[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]
Agar Medium for Differential
Enumeration of Lactic Streptococci

山(shan)東拓(tuo)普生(sheng)物工程有限公司(si) 培養基配方 //ljxinglong.com

Composition per 1170.0mL:

Agar ..................................................................15.0g
Carboxymethylcellulose ...................................15.0g
Calcium citrate..................................................10.0g
Pancreatic digest of casein..................................5.0g
Yeast extract........................................................5.0g
L-Arginine·HCl....................................................5.0g
Casamino acids ...................................................2.5g
K2HPO4.............................................................1.25g
Calcium carbonate solution ........................ 100.0mL
Nonfat milk solution..................................... 50.0mL
Bromcresol Purple solution .......................... 20.0mL
pH 5.9 ± 0.2 at 25°C
Calcium Carbonate Solution:
Composition per 100.0mL:
CaCO3 .................................................................3.0g
Preparation of Calcium Carbonate Solution:
Add CaCO3 to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Nonfat Milk Solution:
Composition per 100.0mL:
Nonfat milk.......................................................11.0g
Preparation of Nonfat Milk Solution: Add
nonfat milk to distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Bromcresol Purple Solution:
Composition per 20.0mL:
Bromcresol Purple ............................................0.02g
Preparation of Bromcresol Purple Solution:
Add Bromcresol Purple to distilled/deionized water
and bring volume to 20.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add agar to 500.0mL
of distilled/deionized water. Gently heat and bring to
boiling. In a separate flask, add carboxymethylcellu
lose and calcium citrate to 500.0mL of distilled/
deionized water. Gently heat while stirring until a
white, turbid suspension is formed. Combine the two
solutions. Add the pancreatic digest of casein, yeast
extract, K2HPO4, casamino acids, and arginine. Mix
thoroughly. Gently heat and bring to boiling. Adjust
pH to 5.6 with 6N HCl. Distribute into screw-capped
bottles in 100.0mL volumes. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Immedi
ately prior to pouring plates, aseptically add 5.0mL
of sterile nonfat milk solution, 10.0mL of sterile cal
cium carbonate solution, and 2.0mL of sterile Brom
cresol Purple solution to each screw-capped bottle.
Mix thoroughly. The pH should be 5.9. Pour into
cold, sterile Petri dishes.
Use: For the cultivation, differentiation, and enu
meration of Lactobacillus lactis, Lactobacillus lactis
subspecies cremoris, and Lactobacillus lactis sub
species diacetylactis. Lactose-fermenting bacteria
such as Lactobacillus lactis subspecies cremoris ap
pear as yellow colonies. Arginine-utilizing bacteria
such as Lactobacillus lactis and Lactobacillus lactis
subspecies diacetylactis appear as purple colonies.
Citrate-utilizing bacteria such as Lactobacillus lactis
subspecies diacetylactis appear as colonies surround
ed by a clear zone.
  版權所有：山東拓普生物工程有限公司 營銷服務熱線：　郵箱：
© 2016-2020 ALL RIGHTS RESERVED.　　
購物車 去頂部