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Aeromonas Medium (Ryan’s Aeromonas Medium)
[所屬分類:培養基配方] [發布時間:2021-10-28] [發布人:網站管理員2] [閱讀次數:] [返回]
Aeromonas Medium
(Ryan’s Aeromonas Medium)
山東拓普生物工(gong)程有(you)限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................12.5g
Na2S2O3 ..........................................................10.67g
Proteose peptone.................................................5.0g
NaCl....................................................................5.0g
Xylose ...............................................................3.75g
L-Lysine·HCl .......................................................3.5g
Yeast extract........................................................3.0g
Sorbitol................................................................3.0g
Bile salts No. 3....................................................3.0g
Inositol ................................................................2.5g
L-Arginine·HCl....................................................2.0g
Lactose................................................................1.5g
Ferric ammonium citrate.....................................0.8g
Bromthymol Blue .............................................0.04g
Thymol Blue .....................................................0.04g
Ampicillin ........................................................5.0mg
pH 8.0 ± 0.1 at 25°C
Source: This medium is available as a dehydrated
powder from Oxoid.
Preparation of Medium: Add components, ex
cept ampicillin, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat
and bring to boiling. Do not autoclave. Cool to 50°C
and aseptically add 5.0mg of ampicillin. Pour into
sterile Petri dishes.
Use: For the isolation and selective differentiation of
Aeromonas hydrophila and other Aeromonas species.
Aeromonas species appear as small (0.5–1.5mm),
dark green colonies with darker centers. The addition
of ampicillin improves the recovery of Aeromonas
spp. Plesiomonas spp. as well as members of the En
terobacteriaceae also grow on this medium.
