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培養基配方

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Acidophilic Bacillus stearothermophilus Broth

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]

Acidophilic Bacillus

stearothermophilus Broth

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Soluble starch....................................................10.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................1.0g

CaCl2·2H2O ........................................................0.5g

MnCl2·4H2O .......................................................0.5g

pH 5.0 ± 0.2 at 25°C

Preparation of Medium: Dissolve all components

except agar in 1.0L of distilled/deionized water. Mix

thoroughly. Gently heat and bring to boiling. Adjust to

pH 5.0. Autoclave for 15 min at 15 psi pressure–121°C.

Precipitate will dissolve after cooling and mixing.

Use: For the cultivation and maintenance of Bacillus

stearothermophilus and other acidophilic Bacillus

species.

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