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Acid Tomato Broth

[所屬分類:培養基配方] [發布時間:2021-10-27] [發布人:網站管理員2] [閱讀次數:] [返回]
Acid Tomato Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................10.0g
Peptone .............................................................10.0g
Yeast extract........................................................5.0g
MgSO4·7H2O......................................................0.2g
MnSO4·4H2O....................................................0.05g
Tomato juice ............................................... 250.0mL
L-Cysteine solution ......................................... 0.5mL
L-Cysteine Solution:
Composition per 10.0mL:
L-Cysteine ...........................................................0.1g
Preparation of L-Cysteine Solution: Add 0.1g
of L-cysteine to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept L-cysteine solution, to distilled/deionized water
and bring volume to 999.5mL. Mix thoroughly. Ad
just pH to 4.8. Autoclave for 15 min at 15 psi pres
sure–121°C. Aseptically add 0.5mL of sterile L
cysteine solution. Mix thoroughly. Aseptically dis
tribute into sterile tubes or flasks.
Use: For the cultivation of a variety of fungi.
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