培養基配方

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Z Agar

[所屬分類:培養基配方] [發布時間:2021-10-25] [發布人:網站管理員2] [閱讀次數:] [返回]

Z Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar....................................................................................16.0

K2HPO4......................................................................................... 5.0g

K2SO4............................................................................................ 2.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O .............................................................................. 0.05g

Acetamide solution ..................................................................40.0mL

pH 7.2 ± 0.2 at 25°C

Acetamide Solution:

Composition per 100.0mL:

Acetamide................................................................................... 10.0g

Preparation of Acetamide Solution: Add acetamide to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except acetamide solu

tion, to distilled/deionized water and bring volume to 960.0mL. Mix

thoroughly. Autoclave for 20 min at 15 psi pressure–121°C. Cool to

70°C. Aseptically add 40.0mL sterile acetamide solution. Mix thor

oughly. Pour into sterile Petri dishes. Dry plates at 37°C for 30 min.

Use: For the isolation of Pseudomonas aeruginosa from milk.