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YTN Medium (Yeast Extract Tryptone NaCl Medium)

[所屬分類:培養基配方] [發布時間:2021-10-25] [發布人:網站管理員2] [閱讀次數:] [返回]
YTN Medium
(Yeast Extract Tryptone NaCl Medium)

山東拓普生物(wu)工程有限公(gong)司 培養基配(pei)方(fang) //ljxinglong.com

Composition per liter:

NaCl ............................................................................................ 30.0g
Agar ............................................................................................ 15.0g
Yeast extract................................................................................ 10.0g
Pancreatic digest of casein.......................................................... 10.0g
Glucose ......................................................................................... 1.0g
Trace elements solution .............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
H3BO3 ......................................................................................... 2.85g
MnCl2·4H2O.................................................................................. 1.8g
Sodium tartrate............................................................................ 1.77g
FeSO4 .......................................................................................... 1.36g
CoCl2·6H2O ................................................................................ 0.04g
CuCl2·2H2O............................................................................... 0.027g
Na2MoO4·2H2O ........................................................................ 0.025g
ZnCl2......................................................................................... 0.021g
pH 7.2 ± 0.2 at 25°C
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to tap water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of ATCC strain 21588.
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