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培養基配方

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YM5 with 10% Sorbitol

[所屬分類:培養基配方] [發布時間:2021-10-22] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Sorbitol...................................................................................... 100.0g

Glucose ....................................................................................... 10.0g

Succinic acid ............................................................................... 10.0g

NaOH ............................................................................................ 6.0g

Peptone.......................................................................................... 2.0g

Yeast extract.................................................................................. 1.0g

Adenine....................................................................................... 0.01g

Uracil .......................................................................................... 0.01g

Yeast nitrogen base solution ..................................................100.0mL

pH 5.8 ± 0.2 at 25°C

Yeast Nitrogen Base Solution:

Composition per 100.0mL:

(NH4)2SO4..................................................................................... 5.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.5g

NaCl .............................................................................................. 0.1g

CaCl2·2H2O................................................................................... 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol .......................................................................................2.0mg

KI ...............................................................................................0.1mg

H3BO3 ........................................................................................0.5mg

ZnSO4·7H2O ..............................................................................0.4mg

MnSO4·4H2O .............................................................................0.4mg

Thiamine·HCl ............................................................................0.4mg

Pyroxidine·HCl ..........................................................................0.4mg

Niacin.........................................................................................0.4mg

Calcium pantothenate ................................................................0.4mg

p-Aminobenzoic acid.................................................................0.2mg

Riboflavin ..................................................................................0.2mg

FeCl3 ..........................................................................................0.2mg

Na2MoO4·4H2O .........................................................................0.2mg

CuSO4·5H2O ............................................................................0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

Source: Yeast nitrogen base is available as a premixed powder from

BD Diagnostic Systems.

Preparation of Yeast Nitrogen Base Solution: Add components

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except yeast nitrogen

base solution, to distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to room temperature. Aseptically add 100.0mL of sterile

yeast nitrogen base solution to the cooled sterile basal medium. Mix

thoroughly. Adjust final pH to 5.8. Distribute into sterile flasks or

tubes.

Use: For the cultivation of a sorbitol-utilizing fungus.

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YM5 with 10% Sorbitol