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YM-1L Broth

[所屬分類:培養基配方] [發布時間:2021-10-22] [發布人:網站管理員2] [閱讀次數:] [返回]
YM-1L Broth

山東(dong)拓普生物(wu)工程有限公司(si) 培養基配(pei)方 //ljxinglong.com

Composition per liter:

Sodium lactate ............................................................................ 30.0g
Mycological peptone .................................................................. 10.0g
Succinic acid............................................................................... 10.0g
NaOH............................................................................................ 6.0g
Yeast extract.................................................................................. 5.0g
Adenine....................................................................................... 0.01g
Uracil .......................................................................................... 0.01g
Yeast nitrogen base solution ..................................................100.0mL
Yeast Nitrogen Base Solution:
Composition per 100.0mL:
(NH4)2SO4 .................................................................................... 5.0g
KH2PO4......................................................................................... 1.0g
MgSO4·7H2O................................................................................ 0.5g
NaCl.............................................................................................. 0.1g
CaCl2·2H2O .................................................................................. 0.1g
DL-Methionine............................................................................. 0.02g
DL-Tryptophan............................................................................. 0.02g
L-Histidine·HCl ........................................................................... 0.01g
Inositol ....................................................................................... 2.0mg
KI ............................................................................................... 0.1mg
H3BO3 ........................................................................................ 0.5mg
ZnSO4·7H2O .............................................................................. 0.4mg
MnSO4·4H2O............................................................................. 0.4mg
Thiamine·HCl ............................................................................ 0.4mg
Pyroxidine·HCl .......................................................................... 0.4mg
Niacin......................................................................................... 0.4mg
Calcium pantothenate ................................................................ 0.4mg
p-Aminobenzoic acid................................................................. 0.2mg
Riboflavin .................................................................................. 0.2mg
FeCl3 .......................................................................................... 0.2mg
Na2MoO4·4H2O ......................................................................... 0.2mg
CuSO4·5H2O............................................................................ 0.04mg
Folic acid ....................................................................................2.0μg
Biotin ..........................................................................................2.0μg
Preparation of Yeast Nitrogen Base Solution: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except yeast nitrogen
base solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 1.0L of sterile yeast nitrogen base solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Saccharomyces cerevisiae.
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