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YGLM with Chalk (Yeast Glucose Litmus Milk with Chalk)

[所屬分類:培養基配方] [發布時間:2021-10-22] [發布人:網站管理員2] [閱讀次數:] [返回]

YGLM with Chalk

(Yeast Glucose Litmus Milk with Chalk)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

CaCO3......................................................................................... 20.0g

Glucose ....................................................................................... 10.0g

Yeast extract.................................................................................. 3.0g

Litmus ......................................................................................... 0.75g

Skim milk...............................................................................100.0mL

Preparation of Medium: Add components, except skim milk, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Add

10.0mL of skim milk. Distribute into tubes. Autoclave for 10 min at 10

psi pressure–115°C. Incubate for 1 week at 30°C to check for sterility

before use.

Use: For the cultivation of Gemella morbillorum.