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YGC Medium with Cysteine (Yeast Extract Glucose Citrate Medium with Cysteine)

[所屬分類:培養基配方] [發布時間:2021-10-22] [發布人:網站管理員2] [閱讀次數:] [返回]
YGC Medium with Cysteine
(Yeast Extract Glucose
Citrate Medium with Cysteine)

山(shan)東拓普生物工程有(you)限公司 培(pei)養基(ji)配方 //ljxinglong.com

Composition per liter:

Glucose ....................................................................................... 10.0g
Peptone........................................................................................ 10.0g
Beef extract ................................................................................. 10.0g
Yeast extract.................................................................................. 5.0g
Ammonium citrate ........................................................................ 5.0g
Sodium acetate.............................................................................. 2.0g
Tween™ 80................................................................................... 1.0g
MgSO4·7H2O ................................................................................ 0.2g
MnSO4·4H2O .............................................................................. 0.05g
L-Cysteine·HCl·H2O solution ..................................................10.0mL
pH 6.5 ± 0.2 at 25°C
L-Cysteine·HCl Solution:
Composition per 10.0mL:
L-Cysteine·HCl·H2O ..................................................................... 0.5g
Preparation of L-Cysteine·HCl Solution: Add L-cyste
ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except for the L-cyste
ine·HCl·H2O, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Aseptically add L-cysteine hydro
chloride solution.
Use: For the cultivation and maintenance of Leuconostoc mesenteroi
des.
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