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YEME Thiostrepton Medium

[所屬分類:培養基配方] [發布時間:2021-10-21] [發布人:網站管理員2] [閱讀次數:] [返回]
YEME Thiostrepton Medium

山東拓普(pu)生物工(gong)程(cheng)有限公(gong)司 培養基配方 //ljxinglong.com

Composition per liter:

Sucrose...................................................................................... 340.0g
Peptone.......................................................................................... 5.0g
Malt extract ................................................................................... 3.0g
Yeast extract.................................................................................. 3.0g
Thiostrepton solution ...............................................................10.0mL
MgCl2·6H2O solution.................................................................2.0mL
Thiostrepton Solution:
Composition per 10.0mL:
Thiostrepton .............................................................................10.0mg
Preparation of Thiostrepton Solution: Add thiostrepton to dis
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
MgCl2·6H2O Solution:
Composition per 100.0mL:
MgCl2·6H2O................................................................................ 50.8g
Preparation of MgCl2·6H2O Solution: Add MgCl2·6H2O to dis
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except thiostrepton so
lution and MgCl2·6H2O solution, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 10.0mL of sterile
thiostrepton solution and 2.0mL of sterile solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Streptomyces lividans.
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