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培養基配方

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Yeast Nitrogen Base Glucose Broth

[所屬分類:培養基配方] [發布時間:2021-10-21] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Yeast nitrogen base ..................................................................25.0mL

Glucose solution ......................................................................25.0mL

pH 5.6 ± 0.2 at 25°C

Yeast Nitrogen Base:

Composition per 500.0mL:

(NH4)2SO4..................................................................................... 5.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.5g

NaCl .............................................................................................. 0.1g

CaCl2·2H2O................................................................................... 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol .......................................................................................2.0mg

H3BO3 ........................................................................................0.5mg

ZnSO4·7H2O ..............................................................................0.4mg

MnSO4·4H2O .............................................................................0.4mg

Thiamine·HCl ............................................................................0.4mg

Pyroxidine·HCl ..........................................................................0.4mg

Niacin.........................................................................................0.4mg

Calcium pantothenate ................................................................0.4mg

p-Aminobenzoic acid.................................................................0.2mg

Riboflavin ..................................................................................0.2mg

FeCl3 ..........................................................................................0.2mg

Na2MoO4·4H2O .........................................................................0.2mg

KI ...............................................................................................0.1mg

CuSO4·5H2O ............................................................................0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

Source: Yeast nitrogen base is available as a premixed powder from

BD Diagnostic Systems.

Preparation of Yeast Nitrogen Base: Add components to dis

tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Filter sterilize.

Glucose Solution:

Composition per 500.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 500.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Aseptically combine 25.0mL of sterile

yeast nitrogen base and 25.0mL of sterile glucose solution. Mix thor

oughly.

Use: For the cultivation and enrichment of yeast from sewage and pol

luted waters.

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Yeast Nitrogen Base Glucose Broth