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Yeast Nitrogen Base with Carbohydrate

[所屬分類:培養基配方] [發布時間:2021-10-20] [發布人:網站管理員2] [閱讀次數:] [返回]
Yeast Nitrogen Base with Carbohydrate

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Carbohydrate................................................................................. 5.0g
(NH4)2SO4 .................................................................................... 5.0g
KH2PO4......................................................................................... 1.0g
MgSO4·7H2O................................................................................ 0.5g
NaCl.............................................................................................. 0.1g
CaCl2·2H2O .................................................................................. 0.1g
DL-Methionine............................................................................. 0.02g
DL-Tryptophan............................................................................. 0.02g
L-Histidine·HCl ........................................................................... 0.01g
Inositol ....................................................................................... 2.0mg
KI ...............................................................................................0.1mg
H3BO3 ........................................................................................0.5mg
ZnSO4·7H2O ..............................................................................0.4mg
MnSO4·4H2O .............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyroxidine·HCl ..........................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl3 ..........................................................................................0.2mg
Na2MoO4·4H2O .........................................................................0.2mg
CuSO4·5H2O ............................................................................0.04mg
Folic acid.....................................................................................2.0μg
Biotin ..........................................................................................2.0μg
pH 5.6 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Asep
tically distribute into tubes or flasks.
Use: For carbohydrate assimilation tests in the characterization and
identification of yeasts.
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