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Yeast Mannitol Agar

[所屬分類:培養基配方] [發布時間:2021-10-20] [發布人:網站管理員2] [閱讀次數:] [返回]
Yeast Mannitol Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 20.0g
Mannitol...................................................................................... 10.0g
Na2HPO4·12H2O........................................................................... 1.2g
KH2PO4....................................................................................... 0.55g
MgSO4·7H2O .............................................................................. 0.25g
NaCl............................................................................................ 0.25g
Yeast extract................................................................................ 0.25g
CaSO4·2H2O ............................................................................ 30.0mg
FeSO4·7H2O............................................................................... 3.5mg
H3BO3 .....................................................................................500.0μg
MnSO4·4H2O ..........................................................................400.0μg
ZnSO4·7H2O ...........................................................................160.0μg
CuSO4·5H2O.............................................................................80.0μg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of Bradyrhizobium japonicum and Rhizobium
leguminosarum.
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