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Yeast Extract Agar

[所屬分類:培養基配方] [發布時間:2021-10-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Yeast Extract Agar

山東(dong)拓普生(sheng)物工程有(you)限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 20.0g
Yeast extract.................................................................................. 1.0g
Buffer solution ...........................................................................2.0mL
pH 6.0 ± 0.2 at 25°C
Buffer Solution:
Composition per 400.0mL:
KH2PO4....................................................................................... 60.0g
Na2HPO4 ..................................................................................... 40.0g
Preparation of Buffer Solution: Add 40.0g of Na2HPO4 to
300.0mL of distilled/deionized water. Mix thoroughly. Add 60.0g of
KH2PO4. Mix thoroughly. Adjust pH to 6.0.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the identification of Histoplasma capsulatum, Blastomyces
dermatitidis, and Coccidioides immitis.
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