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Yeast Carbon Base, 10X (Wickerham Carbon Base Broth)

[所屬分類:培養基配方] [發布時間:2021-10-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Yeast Carbon Base, 10X
(Wickerham Carbon Base Broth)

山東拓普生物工程有(you)限公(gong)司(si) 培(pei)養基配方 //ljxinglong.com

Composition per liter:

Glucose ....................................................................................... 10.0g
KH2PO4......................................................................................... 1.0g
MgSO4·7H2O ................................................................................ 0.5g
NaCl .............................................................................................. 0.1g
CaCl2·2H2O................................................................................... 0.1g
DL-Methionine............................................................................. 0.02g
DL-Tryptophan............................................................................. 0.02g
L-Histidine·HCl ........................................................................... 0.01g
Inositol .......................................................................................2.0mg
H3BO3 ........................................................................................0.5mg
ZnSO4·7H2O ..............................................................................0.4mg
MnSO4·4H2O .............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyridoxine..................................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl3 ..........................................................................................0.2mg
Na2MoO4·4H2O .........................................................................0.2mg
KI ...............................................................................................0.1mg
CuSO4·5H2O ............................................................................0.04mg
Folic Acid ...................................................................................2.0μg
Biotin ..........................................................................................2.0μg
pH 5.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Use: Used as a base to which different nitrogen sources may be added.
For the cultivation and differentiation of bacteria based on their ability
to utilize diverse added nitrogen sources.
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