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Xylose Lactose Tergitol™ 4 (XLT-4)

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Xylose Lactose Tergitol™ 4
(XLT-4)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1004.6mL:

Agar ............................................................................................ 18.0g
Lactose.......................................................................................... 7.5g
Sucrose.......................................................................................... 7.5g
Na2S2O3 ........................................................................................ 6.8g
Lysine............................................................................................ 5.0g
NaCl.............................................................................................. 5.0g
Xylose......................................................................................... 3.75g
Yeast extract.................................................................................. 3.0g
Proteose peptone........................................................................... 1.6g
Ferric ammonium citrate............................................................... 0.8g
Phenol Red.................................................................................. 0.08g
Selective supplement solution ...................................................4.6mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Selective Supplement Solution:
Composition per 100.0mL:
Tergitol™ 4........................................................................ Proprietary
Preparation of Selective Supplement Solution: Available as a
premixed solution.
Preparation of Medium: Add components, except selective sup
plement solution, to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Add 4.6mL of selective supplement solution.
Mix thoroughly. Gently heat while stirring and bring to boiling. Do not
autoclave. Cool to 50°C. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the isolation and identification of salmonellae from clinical,
environmental, and food samples. The presence of the selective agent,
Tergitol™ 4, in this medium inhibits many organisms that can be prob
lematic on other plating media. In addition, biochemical and pH
changes within the medium allow Salmonella spp. (black colonies) to
be differentiated from organisms such as E. coli (yellow colonies) and
Shigella spp. (red colonies). The enhanced selectivity of XLT-4 Agar
reduces the need for further identification procedures, saving time and
money, and results in fewer false presumptive positive colonies when
compared to other Salmonella plating media.
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