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XPS Broth with Thymidine (Thymidine Auxotroph XPS Medium)

[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
XPS Broth with Thymidine
(Thymidine Auxotroph XPS Medium)

山東拓普生物工(gong)程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Potatoes, infusion from............................................................... 40.0g
Sucrose........................................................................................ 15.0g
Peptone ......................................................................................... 5.0g
Glucose ......................................................................................... 4.0g
Casamino acids ............................................................................. 1.0g
Na2HPO4..................................................................................... 0.79g
Thymidine................................................................................ 10.0mg
Ca(NO3)2·4H2O solution .........................................................10.0mL
pH 5.1 ± 0.2 at 25°C
Potatoes, Infusion From:
Composition per 500.0mL:
Potatoes......................................................................................... 4.0g
Preparation of Potatoes, Infusion From: Peel and dice potatoes.
Add 500.0mL of distilled/deionized water. Gently heat and bring to
boiling. Continue boiling for 30 min. Filter through cheesecloth.
Ca(NO3)2·4H2O Solution:
Composition per 10.0mL:
Ca(NO3)2·4H2O ............................................................................ 0.5g
Preparation of Ca(NO3)2·4H2O Solution: Add Ca(NO3)2·4H2O
to distilled/deionized water and bring volume to 10.0mL. Mix thor
oughly. Filter sterilize.
Preparation of Medium: Add components, except Ca(NO3)2·4H2O
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Asep
tically add 10.0mL of sterile Ca(NO3)2·4H2O solution. Mix thorough
ly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Xanthomonas oryzae.
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