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XL Agar Base (Xylose Lysine Agar Base)
[所屬分類:培養基配方] [發布時間:2021-10-15] [發布人:網站管理員2] [閱讀次數:] [返回]
XL Agar Base
(Xylose Lysine Agar Base)
山(shan)東拓普(pu)生(sheng)物工(gong)程有(you)限公(gong)司(si) 培養基配方(fang) //ljxinglong.com
Composition per liter:
Agar ............................................................................................ 13.5g
Lactose .......................................................................................... 7.5g
Sucrose.......................................................................................... 7.5g
L-Lysine......................................................................................... 5.0g
NaCl .............................................................................................. 5.0g
Xylose ........................................................................................... 3.5g
Yeast extract.................................................................................. 3.0g
Phenol Red.................................................................................. 0.08g
Thiosulfate-citrate solution ......................................................20.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di
agnostic Systems.
Thiosulfate-Citrate Solution:
Composition per 100.0mL:
Na2S2O3 ...................................................................................... 34.0g
Ferric ammonium citrate............................................................... 4.0g
Preparation of Thiosulfate-Citrate Solution: Add components
to distilled/deionized water and bring volume to 100.0mL. Mix thor
oughly.
Preparation of Medium: Add components, except thiosulfate-cit
rate solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Gently heat while stirring and bring to boil
ing. Distribute into tubes or flasks. Autoclave for 10 min at 14 psi pres
sure–118°C. Cool to 55°C. Aseptically add 20.0 mL of the sterile thio
sulfate-citrate solution. Mix thoroughly. Pour into sterile Petri dishes or
leave in tubes.
Use: For the isolation, cultivation, and differentiation of enteric patho
gens. Nonfermenting xylose/lactose/sucrose bacteria appear as red col
onies. Xylose-fermenting, lysine-decarboxylating bacteria appear as
red colonies. Xylose-fermenting, lysine-nondecarboxylating bacteria
appear as opaque yellow colonies. Lactose- or sucrose-fermenting bac
teria appear as yellow colonies.
