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Wort HiVeg Agar

[所屬分類:培養基配方] [發布時間:2021-10-14] [發布人:網站管理員2] [閱讀次數:] [返回]
Wort HiVeg Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 15.0g
Malt extract................................................................................. 15.0g
Maltose ..................................................................................... 12.75g
Dextrin ........................................................................................ 2.75g
NH4Cl ........................................................................................... 1.0g
K2HPO4......................................................................................... 1.0g
Plant peptone............................................................................... 0.78g
pH 4.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Boil for 1 min with mixing. Distribute into tubes or flasks. Au
toclave for 15 min at 15 psi pressure–121°C. Do not overheat, as this will
result in hydrolysis of the agar. An additional 5.0g of agar can be used to
make a firmer agar. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and enumeration of yeasts. The low pH of the
agar selectively inhibits bacterial growth.
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