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Wort Agar 6°Brix

[所屬分類:培養基配方] [發布時間:2021-10-14] [發布人:網站管理員2] [閱讀次數:] [返回]
Wort Agar 6°Brix

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Malt........................................................................................... 250.0g
Agar ............................................................................................ 20.0g
Preparation of Medium: Add 250.0g of ground malt to tap water
and bring volume to 1.0L. Mix thoroughly. Gently heat to 55°–60°C.
Maintain at 55°–60°C for 1.5–2.0 hr while mixing regularly. Elevate
temperature to 80°C and maintain for 10 min with thorough mixing.
Cool the wort to 25°C and filter through a linen bag. Adjust the con
centration of sugars to 6° Brix (specific gravity of 1.024 at 24°C). Add
20.0g of agar. Gently heat and bring to boiling. Mix thoroughly. Dis
tribute into tubes or flasks. Autoclave for 30 min at 3 psi pressure–
105°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Rhodococcus erythropo
lis.
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