<bdo id='0b8uy'><sup id='0b8uy'><div id='0b8uy'><bdo id='0b8uy'></bdo></div></sup></bdo>

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Winogradsky’s N-Free Medium

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓(tuo)普(pu)生物工(gong)程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 20.0g

CaCO3 ........................................................................................ 5.0mg

Sugar solution ........................................................................100.0mL

Concentrated salt solution..........................................................5.0mL

pH 7.2 ± 0.2 at 25°C

Sugar Solution:

Composition per 100.0mL:

Sucrose or glucose ...................................................................... 10.0g

Preparation of Sugar Solution: Add sucrose or glucose to 100.0mL

of distilled/deionized water. Mix thoroughly. Autoclave for 10 min at 10 psi

pressure–115°C. Cool to 50°C.

Concentrated Salt Solution:

Composition per liter:

KH2PO4....................................................................................... 50.0g

MgSO4·7H2O.............................................................................. 25.0g

NaCl............................................................................................ 25.0g

FeSO4·7H2O.................................................................................. 1.0g

MnSO4·4H2O................................................................................ 1.0g

Na2MoO4·4H2O ............................................................................ 1.0g

Preparation of Concentrated Salt Solution: Add components

to tap water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except sugar solution,

to distilled/deionized water and bring volume to 900.0mL. Mix thor

oughly. Gently heat and bring to boiling. Distribute into tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add sugar solution. Adjust pH to 7.2. Mix thoroughly. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Azomonas insignis.