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Wilkins-Chalgren Anaerobic HiVeg Agar Base with Blood

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Wilkins-Chalgren Anaerobic HiVeg Agar Base
with Blood

山東拓普生物工程有限公司 培養(yang)基配方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 10.0g
Plant hydrolysate ........................................................................ 10.0g
Plant peptone .............................................................................. 10.0g
NaCl.............................................................................................. 5.0g
Yeast extract.................................................................................. 5.0g
L-Arginine..................................................................................... 1.0g
Glucose ......................................................................................... 1.0g
Sodium pyruvate........................................................................... 1.0g
Fe4(P2O7)3·H2O ......................................................................... 5.0mg
Menadione ................................................................................. 0.5mg
Defibrinated blood...................................................................50.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without blood, is available as a premixed pow
der from HiMedia.
Preparation of Medium: Add components, except defibrinated
blood, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–
55°C. Aseptically add 50.0mL of defibrinated blood. Mix thoroughly.
Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of nonsporulating anaerobes. For the cultivation
and maintenance of anaerobic bacteria. For standardized antimicrobic
susceptibility testing to determine the minimum inhibitory concentra
tions of antimicrobics for anaerobic bacteria.
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