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Wilkins-Chalgren Anaerobe Agar with GN Supplement

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Wilkins-Chalgren Anaerobe Agar
with GN Supplement

山東(dong)拓(tuo)普生物(wu)工程有限公司 培養基配(pei)方 //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 10.0g
Pancreatic digest of casein.......................................................... 10.0g
Gelatin peptone........................................................................... 10.0g
NaCl.............................................................................................. 5.0g
Yeast extract.................................................................................. 5.0g
Glucose ......................................................................................... 1.0g
L-Arginine ..................................................................................... 1.0g
Sodium pyruvate........................................................................... 1.0g
Hemin ........................................................................................ 5.0mg
Menadione ................................................................................. 0.5mg
Defibrinated blood...................................................................50.0mL
GN anaerobe selective supplement..........................................20.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
GN Anaerobe Selective Supplement
Composition per 20.0mL:
Nalidixic acid........................................................................... 10.0mg
Hemin ........................................................................................ 5.0mg
Sodium succinate....................................................................... 2.5mg
Vancomycin ............................................................................... 2.5mg
Menadione ................................................................................. 0.5mg
Preparation of GN Anaerobe Selective Supplement: Add
components to distilled/deionized water and bring volume to 20.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except defibrinated
blood and GN anaerobe selective supplement, to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL
of GN anaerobe selective supplement and 50.0mL of defibrinated
blood. Bring volume to 1.0L with distilled/deionized water. Mix thor
oughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation of Gram-negative anaerobes.
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