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Wheat Peptone Agar

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]
Wheat Peptone Agar

山東拓普生物工(gong)程有限(xian)公司(si) 培(pei)養基配方 //ljxinglong.com

Composition per 750.0mL:

Agar ............................................................................................ 20.0g
Peptone........................................................................................ 20.0g
Yeast extract................................................................................ 10.0g
Glycerol ...................................................................................... 10.0g
Wheat grain extract................................................................500.0mL
pH 6.8–7.0 at 25°C
Wheat Grain Extract:
Composition per 500.0mL:
Whole wheat ............................................................................... 30.0g
Preparation of Wheat Grain Extract: Add whole wheat to tap wa
ter and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring
to boiling. Continue boiling for 60 min. Filter through several thicknesses
of cheesecloth padded with cotton.
Preparation of Medium: Add agar, peptone, yeast extract, and
glycerol to whole grain extract filtrate. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or aseptically distribute into sterile tubes.
Use: For the cultivation and utilization of Septoria nodorum.
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