培養基配方

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Wesley Broth

[所屬分類:培養基配方] [發布時間:2021-10-13] [發布人:網站管理員2] [閱讀次數:] [返回]

Wesley Broth

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Tryptose ...................................................................................... 20.0g

Bicine (N,N-bis-2-[hydroxyethyl]glycine) buffer....................... 10.0g

NaCl .............................................................................................. 5.0g

Yeast extract.................................................................................. 2.5g

Agar .............................................................................................. 1.0g

FeSO4 .......................................................................................... 0.25g

Na2S2O5 ...................................................................................... 0.25g

Sodium pyruvate ......................................................................... 0.25g

Antibiotic solution ...................................................................10.0mL

Alkaline hematin solution........................................................6.25mL

Antibiotic Solution:

Composition per 10.0mL:

Rifampin ................................................................................... 0.025g

Cefsulodin................................................................................6.25mg

Polymyxin B sulfate............................................................... 20,000U

Preparation of Antibiotic Solution: Add components to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Alkaline Hematin Solution:

Composition per 10.0mL:

Hemin........................................................................................ 0.032g

NaOH (0.15N solution)............................................................10.0mL

Preparation of Hemin Solution: Add hemin to 10.0mL of NaOH

solution. Mix thoroughly. Autoclave for 30 min at 5 psi pressure–108°C.

Cool to 25°C.

Preparation of Medium: Add components, except antibiotic solu

tion and alkaline hematin solution, to distilled/deionized water and

bring volume to 983.75mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add 10.0mL of sterile antibiotic solution and 6.25mL

of sterile alkaline hematin solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes. Use medium immediately or store overnight

at 4°C.

Use: For the enrichment of Campylobacter species from foods.