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Waxy Maize Starch Medium

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Waxy Maize Starch Medium

山東拓普生物工程有限公司 培養基配方(fang) //ljxinglong.com

Composition per liter:

Agar ............................................................................................ 20.0g
Waxy maize starch ........................................................................ 5.0g
Pancreatic digest of casein............................................................ 5.0g
Yeast extract.................................................................................. 5.0g
CoCl2·6H2O .................................................................................. 0.1g
CaCl2·2H2O .................................................................................. 0.1g
Maltose solution.....................................................................100.0mL
pH 6.7 ± 0.2 at 25°C
Maltose Solution:
Composition per 100.0mL:
Maltose ....................................................................................... 10.0g
Preparation of Maltose Solution: Add maltose to distilled/deion
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster
ilize.
Preparation of Medium: Add components, except maltose solu
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 6.7. Distrib
ute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically add maltose solution. Pour into sterile Petri dishes or leave
in tubes.
Use: For the cultivation and maintenance of Bacillus species.
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