培養基配方

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Wang’s Transport Storage Medium

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓普生物工(gong)程有限(xian)公司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein.......................................................... 10.0g

Peptic digest of animal tissue...................................................... 10.0g

NaCl .............................................................................................. 5.0g

Agar .............................................................................................. 4.0g

Yeast extract.................................................................................. 2.0g

Glucose ......................................................................................... 1.0g

NaHSO3 ........................................................................................ 0.1g

Sheep blood, defibrinated ......................................................100.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep

blood. Sheep blood may be replaced by 50.0mL of horse blood and

50.0mL of sterile distilled/deionized water. Mix thoroughly. Aseptical

ly distribute into sterile screw-capped tubes in 4.0mL volumes. Allow

tubes to cool in an upright position.

Use: For the cultivation, transport, and maintenance of Campylobacter

species from foods.