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Wagatsuma HiVeg Agar Base with Red Blood Cells

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Wagatsuma HiVeg Agar Base with Red Blood Cells

山東(dong)拓(tuo)普生物工程有限(xian)公司 培養基(ji)配方 //ljxinglong.com

Composition per 1050.0mL:

NaCl............................................................................................ 70.0g
Agar ............................................................................................ 15.0g
Plant peptone .............................................................................. 10.0g
Mannitol...................................................................................... 10.0g
K2HPO4......................................................................................... 5.0g
Yeast extract.................................................................................. 3.0g
Crystal Violet............................................................................. 1.0mg
Red blood cells ........................................................................50.0mL
pH 8.0 ± 0.2 at 25°C
Source: This medium, without red blood cells, is available as a pre
mixed powder from HiMedia.
Red Blood Cells:
Composition per 100.0mL:
Blood, human or rabbit..........................................................100.0mL
Preparation of Red Blood Cells: Mix freshly drawn human or
rabbit blood with anticoagulant and an equal volume of sterile 0.85%
saline solution. Centrifuge cells at 4000 × g at 4°C for 15 min. Pour off
saline and wash two more times with sterile saline. After last wash,
pour off saline and resuspend cells to their original volume.
Preparation of Medium: Add components, except blood, to dis
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad
just pH to 8.0. Place in a steam bath for 30 min. Do not autoclave. Cool
to 45°–50°C. Add 50.0mL of washed red blood cells. Mix thoroughly.
Pour into sterile Petri dishes. Dry plates before using.
Use: For the cultivation and detection of thermostable hemolysin of
Vibrio parahaemolyticus by the Kanagawa reaction.
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