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VRE Agar

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]

VRE Agar

山東拓普生(sheng)物工程有限公司(si) 培(pei)養基配方 //ljxinglong.com

Composition per 1004.0mL:

Tryptone...................................................................................... 20.0g

Agar ............................................................................................ 10.0g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 5.0g

Sodium citrate............................................................................... 1.0g

Esculin .......................................................................................... 1.0g

Ferric ammonium citrate............................................................... 0.5g

NaN3 ........................................................................................... 0.15g

Selective supplement solution ...................................................4.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Selective Supplement Solution:

Composition per 4.0mL:

Meropenum................................................................................ 1.0mg

Vancomycin ............................................................................... 6.0mg

Preparation of Selective Supplement Solution: Add components

to distilled/deionized water and bring volume to 4.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except selective sup

plement solution, to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asep

tially add 4.0mL selective supplement solution. Mix thoroughly . Pour

into sterile Petri dishes.

Use: For the isolation of vancomycin resistant enterococci (VRE)

from clinical samples. Nonresistant enterococci containing the Van C

genes will not grow on this medium.The selective supplement sup

presses growth of Gram-negative bacteria and E. gallinarum. The

medium contains an indicator system to detect the growth of esculin

hydrolyzing organisms. Enterococci produce black zones around the

colonies from the formation of black iron phenolic compounds derived

from esculin-hydrolyis products and ferrous iron.